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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a crucial role in endorsing cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two additional splice variants ended up noted but in-depth nucleotide sequence data isn't nonetheless obtainable.
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Blocking peptides are peptides that bind specifically for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope regarded from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible effects are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best success are...
Antibodies sure to the blocking peptide no more bind for the epitope within the focus on protein. By evaluating the staining within the blocked antibody versus the antibody alone, one can see which staining is unique.
Concentrate on Facts Cyclin E belongs to your hugely conserved cyclin household, whose users are characterised by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of every mitotic event. Cyclin E sorts a fancy with and website capabilities as a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is particularly degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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